JAHA: Tell us about the key findings from your recent article in JAHA.
Dr. Iso: PPARg induces
expression of FABP4 and FAT/CD36 in capillary endothelial cells to promote FA
transport into heart. This system is activated in the postprandial state after
prolonged fasting.
JAHA: What are the major implications of this work?
Dr. Iso: Our findings identify capillary endothelium as promising target
cells for controlling metabolism of energy substrates in the heart. That is, our
demonstration in vivo opens a new
research area on the role of trans-endothelial material transport system for the
treatment and prevention of cardio-metabolic disease, such as heart failure and
metabolic syndrome.
JAHA: How did you get the idea to do this study?
Dr. Iso: We
found that FABP4 is strongly expressed in capillary endothelial cells, by
immunohistochemistry. FABP4 is cytosolic fatty acid chaperon and one of the
most famous PPARg target genes. These findings led me to come up with the
idea.
JAHA: What was your biggest obstacle in completing this study?
Dr. Iso: While
we were working on this project, a similar study was published in Journal of
Clinical Investigation 2009 by Kanda et al.
Adding new data and new story was tough.
JAHA: What was your most unexpected finding?
Dr. Iso: Uptake
of 125I-BMIPP in hearts was comparable between wild-type and
endothelial PPARg KO mice during fasting. We expected remarkable
difference. We finally found difference of fatty acid uptake after olive oil
gavage.
JAHA: What do you plan to do next, based on these current findings?
Dr. Iso: We
will estimate function of endothelial FABP4 and FAT/CD36 by generating
endothelial-specific KO mice.
JAHA: What do you like to do in your free time?
Dr. Iso: I
play with my kids. Ski,skate, watching movies, etc.
JAHA: What is your favorite sports team or musical group?
Profile originally published February 19, 2013
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